Nuhamin Kasu Assefa

My name is Nuhamin Kasu Assefa, and I am a researcher in plant molecular biology and biotechnology at the Bio and Emerging Technology Institute (BETin), Addis Abeba, Ethiopia. My work focuses on developing molecular tools to support crop improvement, with particular emphasis on underutilized crops such as enset, which play a crucial role in food security in Ethiopia.
Enset (Ensete ventricosum), often referred to as the “false banana,” is a staple crop cultivated mainly in southern Ethiopia, but with a great potential through the country. It is highly valued for its resilience to drought and its ability to provide a stable and reliable food source for millions of people, especially during periods of climate stress or crop failure.

In 2026 I was selected to conduct a Short Research Stay of 3 months (February to May) at the Plant Systems Biotechnology center of VIB in Ghent, Belgium. In the lab of Plant Genome Editing, learning from Prof. Dr. Thomas Jacobs, I focused on optimizing protoplast-based transfection systems and DNA delivery methods for enset. The aim was to establish reliable protocols for gene expression analysis in this crop, which currently lacks well-developed molecular tools. This is an important step toward enabling more advanced genetic and biotechnological applications in enset research. I worked with a range of molecular techniques, including protoplast isolation, PEG-mediated transfection, CTAB-based DNA extraction, and PCR-based validation. I could refine complex protocols and better understand the challenges involved in adapting these methods to a less-studied species.

One of the most valuable aspects of this experience was the opportunity to go beyond basic training and engage deeply in experimental work. I was able to troubleshoot, optimize, and adapt protocols in real time, which helped me build both technical confidence and independence. Working in a well-equipped and collaborative environment also gave me insight into how cutting-edge molecular research is organized and conducted. In my view, research exchanges like this are essential for connecting global scientific advances with local challenges. They equip researchers with practical skills and experience that can be directly applied in their home institutions, ultimately contributing to more effective and sustainable research systems.

When I return to my home institution, my goal is to implement and adapt these optimized protocols to support ongoing crop improvement efforts, particularly for enset. I also place strong emphasis on knowledge sharing: I plan to train colleagues and students, helping to build local expertise in molecular techniques and strengthen our research capacity.